Statistical scanning method for the optimization of gradient elution high performance liquid chromatography |
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Authors: | Q -S Wang R -Y Gao B -W Yan D -P Fan |
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Institution: | (1) National Laboratory of Elemento-Organic Chemistry, Nankai University, 300071 Tianjin, P.R.China |
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Abstract: | Summary A computer-assisted method is presented for the optimization of separation in gradient elution reversed-phase HPLC. The method is based on a polynomial estimation from nine preliminary experiments according to a two-factor (initial solvent composition C and gradient time T) rectangular design. This is followed by a two-dimension computer scanning technique. Resolution is used as the selection criterion. Good agreement was obtained between predicted data and experimental results. |
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Keywords: | Column liquid chromatography Optimal mobile phase Gradient elution Microcomputer |
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