Sensitive chemiluminescence method for the determination of glutathione, l-cysteine and 6-mercaptopurine |
| |
| Authors: | Ping Yang Yonghong Chen Qiyong Zhu Fengwu Wang Lun Wang Yongxin Li |
| |
| Affiliation: | 1. Department of Chemistry, Huainan Normal University, Huainan, 232001, People’s Republic of China
2. Anhui Key Laboratory of Functional Molecular Solids, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 24100, People’s Republic of China
|
| |
| Abstract: | Glutathione (GSH), l-cysteine (l-Cys) and 6-mercaptopurine (6-MP) inhibit the CL reaction of luminol–H2O2 catalyzed by gold colloids. In order to explore this, GSH, l-Cys and 6-MP were injected into the chemiluminescence system of luminol and H2O2 catalyzed by gold colloids. The results showed that gold colloids interact with GSH, l-Cys and 6-MP and decrease the CL emission. Based on this phenomenon, a simple, sensitive and convenient flow injection CL method was developed for the determination of GSH, l-Cys and 6-MP. This method provides a novel, and effective CL assay for GSH, l-Cys and 6-MP that has been applied to the determination of GSH in human serum. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
