Incorporation of impurity to a tetragonal lysozyme crystal |
| |
| Institution: | 1. Advanced Science Research Center, Japan Atomic Energy Research Institute, 2-4 Shiraka-shirane, Tokai-mura, Naka-gun, Ibaraki 319-1195, Japan;2. Institute for Materials Research, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan;3. Faculty of Science and Engineering, Ritsumeikan University, Nozihigashi 1-1-1, Kusatsu-shi, Shiga 525-8577, Japan;4. Faculty of Science, Tohoku University, Aza-aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan;1. Department of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut 06269;2. Institute of Material Science, University of Connecticut, Storrs, Connecticut 06269;1. Institute of Physics, Polish Academy of Sciences, al. Lotników 32/46, 02-668 Warsaw, Poland;2. Faculty of Mathematics and Natural Sciences, Card. S. Wyszynski University, ul. Dewajtis 5, 01-815 Warsaw, Poland;3. Institute of Electronics, Bulgarian Academy of Sciences, 72 Tzarigradsko Chaussee Blvd, 1784 Sofia, Bulgaria;4. R. Kaischew Institute of Physical Chemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Block 11, 1113 Sofia, Bulgaria;1. Department of Chemistry, 219 Parkman Avenue, Chevron Science Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15213 USA;2. Department of Neurological Surgery, 200 Lothrop Street, UPMC Presbyterian Hospital, Pittsburgh, Pennsylvania, 15213 USA;1. The European Synchrotron Radiation Facility, BP 220, F-38043 Grenoble Cedex 9, France;2. Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525AJ Nijmegen, The Netherlands;3. DESY Nanolaboratory, Deutsches Elektronen-Synchrotron (DESY), Notkestraße 85, D-22607 Hamburg, Germany;1. Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, Connecticut 06269;2. Physical Sciences, Inc., Andover, Massachusetts 01810 |
| |
| Abstract: | Concentration of a phosphor-labeled impurity (ovalbumin) incorporated into protein (hen egg white lysozyme) crystals during growth was measured by fluorescence.This technique enabled us to measure the local impurity concentration in a crystal quantitatively. Impurity concentration increased with growth rate, which could not be explained by two conventional models (equilibrium adsorption model and Burton–Prim–Slichter model); a modified model is proposed. Impurity concentration also increased with the pH of the solution. This result is discussed considering the electrostatic interaction between the impurity and the crystallizing species. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
