Separation of siderophores by capillary electrophoresis |
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| Institution: | 1. CEA, DEN, DPC, SECR, Laboratoire d''Etude du Comportement des Bétons et des Argiles, F-91191 Gif-sur-Yvette, France;2. Paris Est University, Navier Institute (UMR8205 CNRS/ENPC/IFSTTAR), 2 allée Kepler, F-77420 Marne La Vallée, France;1. Dep. of Mechanical and Mining Engineering, Escuela Politécnica Superior de Jaén, University of Jaén, Campus Las Lagunillas s/n, 23071 Jaén, Spain;2. Dep. of Electronic Engineering and Automatics, Escuela Politécnica Superior de Jaén, University of Jaén, Campus Las Lagunillas s/n, 23071 Jaén, Spain |
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| Abstract: | Capillary electrophoresis (CE) was applied as a fast method of siderophore separation. Siderophores are iron binding and regulating cell products, which facilitate iron transport into cells. A fast and efficient method of siderophore analysis is important for better understanding of the iron pathways in a sea environment or marine organisms. The best results of CE analysis were obtained using free zone CE in 25 mM phosphate buffer at basic pH using a constant voltage of 20 kV. Under these conditions it was possible to detect the presence of siderophores in seawater. |
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