Crystallization with oils: a new dimension in macromolecular crystal growth |
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| Affiliation: | 1. Grupo de Inmunología y Epidemiología Molecular, GIEM, Facultad de Salud, Universidad Industrial de Santander, Bucaramanga, Colombia;2. Grupo de Ciencias Cardiovasculares, Fundación Cardiovascular de Colombia, Floridablanca, Santander, Colombia;3. Instituto de Parasitología y Biomedicina López-Neyra, IPBLN-CSIC, Granada, Spain;1. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, China;2. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, China;1. Private Practice, Ottawa, Ontario, Canada;2. Department of Diagnostic Imaging, Hospital for Sick Children, Toronto, Ontario, Canada;3. Department of Medical Imaging, University of Toronto; Department of Diagnostic Imaging, Hospital for Sick Children, Toronto, Ontario, Canada;4. Discipline of Orthodontics, Faculty of Dentistry, University of Toronto; Division of Orthodontics, Hospital for Sick Children, Toronto, Ontario, Canada;5. Division of Biostatistics, Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada;1. School of Pharmaceutical Science, Shandong University, 44 West Wenhua Road, Jinan, Shandong Province 250012, PR China;2. Key Lab of Colloid & Interface Chemistry, Shandong University, Ministry of Education, 250100, PR China;3. School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia;1. Department of Chemistry, Faculty of Science, University of Kragujevac, R. Domanovića 12, 34000 Kragujevac, Serbia;2. Faculty of Technical Sciences, University of Priština, Kneza Miloša 7, Kosovska Mitrovica 38220 Serbia;3. Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia |
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| Abstract: | The crystal growth of biological macromolecules is a complicated process involving numerous parameters. This paper presents an approach which employs the use of oil as a major aid to crystal growth, and which has opened up a new dimension in the field of macromolecular crystallization. The presence of oil is a parameter which can contribute to the accuracy, the cleanliness and to the increase in the reproducibility of the experiments. Furthermore, the oil has a role in the protection of the trials during the course of their duration and in maintaining the stability of the resulting crystals. The use of oil also applies to the crystallization of membrane proteins. The results of a wide range of experiments which exploit the presence of oil to abet macromolecular crystal growth using both vapour diffusion and microbatch are presented. |
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