| 用硼酸作为显色剂光度法测定阿昔洛韦 |
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| 引用本文： | 李晶,汪瑾,魏献军,李全民. 用硼酸作为显色剂光度法测定阿昔洛韦[J]. 分析试验室, 2010, 29(3) |
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| 作者姓名： | 李晶 汪瑾 魏献军 李全民 |
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| 作者单位： | 河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,新乡,453007;河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,新乡,453007;河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,新乡,453007;河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,新乡,453007 |
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| 基金项目： | 河南省自然科学基金(082300420210;082300420220)项目资助 |
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| 摘 要： | 在pH12.00的缓冲溶液中,阿昔洛韦(ACV)与H3BO3形成组成比为1∶1的反应产物,其最大吸收波长λmax=289 nm,ACV的质量浓度在0.48～57.6 mg/L范围内与吸光度成良好关系,线性回归方程A=-0.01796+0.01624ρ,相关系数r=0.9990,表观摩尔吸光系数ε=3.7×103L.mol-1.cm-1,回收率为99.9%～101.6%。据此建立了测定ACV的新方法,能够直接用于药物样品中ACV的测定。
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| 关 键 词： | 硼 阿昔洛韦 分光光度法 |
Spectrophotometric determination of acyclovir with boric acid as a chromogenic agent |
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| LI Jing,WANG Jin,WEI Xian-jun,LI Quan-min. Spectrophotometric determination of acyclovir with boric acid as a chromogenic agent[J]. Chinese Journal of Analysis Laboratory, 2010, 29(3) |
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| Authors: | LI Jing WANG Jin WEI Xian-jun LI Quan-min |
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| Affiliation: | College of Chemistry and Environmental Science;Henan Normal University;Henan Key Laboratory for Environmental Pollution Control;Xinxiang 453007 |
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| Abstract: | A simple and sensitive spectrophtometric method is described for the determination of acyclovir (ACV). The method is based on a complex reaction to measure the product formed by the reaction of acyclovir with boric acid in pH 12.0 buffer solution. The stoichiometric ratio of the product is 1∶1, and its maximum absorption wavelength is at 289 nm with an apparent absorptivity ε of 3.7×10~3 L/(mol·cm). The Beer's law is obeyed in the range of 0.48～57.6 mg/L for acyclovir. The linear regression equation is A=-0.01796+0.01624ρ (mg/L), with a linear correlation coefficient of 0.9990. The detection limit is 0.13 mg/L, and average recovery is over 99%. This proposed method has been successfully applied to the determination of acyclovir with satisfactory results. |
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| Keywords: | Boron Acyclovir Spectrophotometry |
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