Amperometric sensor based on the alkaline phosphatase activity |
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Affiliation: | Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid Spain |
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Abstract: | The construction and response of an immobilized enzyme electrode as an amperometric sensor are described. Alkaline phosphatase was covalently bonded to a nylon filter mesh using glutaraldehyde and bovine serum albumin (BSA) as crosslinkers. This modified membrane was then attached to the surface of a glassy-carbon (GC) electrode. Substrate mass transport and enzymatic catalysis control were investigated in a rotating disk electrode. Various response characteristics and kinetic parameters were evaluated and are compared to those of a previously reported amperometric alkaline phosphatase electrode. |
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