Amperometric sensor based on the alkaline phosphatase activity

The construction and response of an immobilized enzyme electrode as an amperometric sensor are described. Alkaline phosphatase was covalently bonded to a nylon filter mesh using glutaraldehyde and bovine serum albumin (BSA) as crosslinkers. This modified membrane was then attached to the surface of a glassy-carbon (GC) electrode. Substrate mass transport and enzymatic catalysis control were investigated in a rotating disk electrode. Various response characteristics and kinetic parameters were evaluated and are compared to those of a previously reported amperometric alkaline phosphatase electrode.