Use of NOESY for estimation of coupling constants in the DNA backbone |
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| Affiliation: | 1. College of Resources and Environmental Sciences, Southwest University, Chongqing 400715, China;2. College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China;3. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China;4. Plant Genome Mapping Laboratory, University of Georgia, Athens, GA 30605, USA;1. Johannes Gutenberg-Universität, 55099 Mainz, Germany;2. Helmholtz-Institut Mainz, 55099 Mainz, Germany;3. Nuclear Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States;4. Department of Physics, University of California at Berkeley, CA 94720-7300, United States |
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| Abstract: | We demonstrate here the use of the two-dimensional NOESY technique for measurement of the most elusive coupling constants, J(H4′-H5′), J(H4′-H5″), J(H3′-H4′) and J(H3′-P) in long DNA segments. The band selective BURP family of pulses has been used in the NOESY pulse sequence for achieving high resolution in the spectra and the coupling constants have been estimated by simulating cross-sections through H1′-H4′ and H8-H3′ cross-peaks in two illustrative cases. |
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