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Fluorescence quenching of thionine by reduced nicotinamide adenine dinucleotide
Affiliation:1. Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea;2. Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejeon 34113, Republic of Korea;3. Department of Materials Science and Engineering, Institute of Nanobiotechnology, Johns Hopkins University (JHU), Baltimore, MD 21218, USA;4. Department of Urology, Asan Medical Center (AMC), University of Ulsan College of Medicine, Seoul 05505, Republic of Korea;1. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Box 92, 1 Beichen West Rd, Chaoyang District, Beijing 100101, China;2. Muséum national d''Histoire naturelle, Dépt. Systématique & Evolution, CP 50 Entomologie, UMR 7205, ISYEB, 75231 Paris Cedex 05, France;3. Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, State Key Laboratory of Palaeobiology and Stratigraphy, 39 East Beijing Rd., Nanjing 210008, China;4. P.O. Box 4680, Chongqing 400015, China
Abstract:Reduced nicotinamide adenine dinucleotide (NADH) is shown to quench the fluorescence of thionine. Quenching of thionine is extremely efficient with a half quenching concentration of only 16.1 × 10−6 M NADH. A Stern—Volmer plot is linear over the NADH concentration range from 1 to 20μM. The corresponding Stern—Volmer quenching constant is 6.2 × 104 M−1 and the limit of detection for NADH measurements is 1.6 × 10−6 M. Process of quenching is attributed to the formation of an exciplex between thionine and NADH. Potential analytical features of this system are discussed.
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