| 高效液相色谱法快速检测猪组织中泰妙菌素残留 |
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| 引用本文： | 程林丽,张素霞,沈建忠,王战辉,李彦伸,冯培生. 高效液相色谱法快速检测猪组织中泰妙菌素残留[J]. 分析化学, 2009, 37(5) |
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| 作者姓名： | 程林丽 张素霞 沈建忠 王战辉 李彦伸 冯培生 |
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| 作者单位： | 中国农业大学动物医学院,北京,100193 |
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| 摘 要： | 应用高效液相色谱法测定猪组织中泰妙菌素残留.试样经磷酸盐缓冲液(pH 8.0)-乙酸乙酯混合提取,离心后加0.1%酒石酸,40 ℃旋转蒸发,除尽有机相,加正已烷除脂,HLB柱净化后,氮气吹干,0.0125%甲酸溶解后HPLC检测.流动相为乙腈-0.0125%甲酸(42∶ 58,V/V).紫外检测波长为210 nm.对猪肉(猪肝)进行15(25)～500 μg/kg的药物添加回收实验,结果表明,泰妙菌素的平均回收率为80.1%～92.7%; 日内相对标准偏差为2.0%～10.2%;日间相对标准偏差为5.9%～13.9%.按信噪比S/N=3计算,猪肉和猪肝中泰妙菌素的检出限(LOD)分别为4.5和7.5 μg/kg.
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| 关 键 词： | 泰妙菌素 猪肉 猪肝 高效液相色谱 |
A Rapid High Performance Liquid Chromatographic Method for Determination of Tiamulin Residue in Swine Tissues |
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| CHENG Lin-Li,ZHANG Su-Xia,SHEN Jian-Zhong,WANG Zhan-Hui,LI Yan-Shen,FENG Pei-Sheng. A Rapid High Performance Liquid Chromatographic Method for Determination of Tiamulin Residue in Swine Tissues[J]. Chinese Journal of Analytical Chemistry, 2009, 37(5) |
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| Authors: | CHENG Lin-Li ZHANG Su-Xia SHEN Jian-Zhong WANG Zhan-Hui LI Yan-Shen FENG Pei-Sheng |
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| Affiliation: | Veterinary Medicine College of China Agricultural University;Beijing 100193 |
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| Abstract: | A rapid method has been developed for the determination of tiamulin(TML) residue in swine tissues by high performance liquid chromatography.The samples were extracted with phosphate buffer-ethyl acetate solution,and then centrifuged by 5000 rpm for 10 min,0.1% fartrate solution was added in the supernatant obtained with liguid-liquid-liquid extraction,and the organic phase was eveporated.After fats having been removed with hexan,further purification was performed with Oasis HLB solid phase extraction column... |
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| Keywords: | Tiamulin pork swine liver high performance liquid chromatography |
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