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| 光谱法研究高铁肌红蛋白活性中心与咪唑的配位反应 | |
| 引用本文： | 周华伟,曹洪玉,唐 乾,李进京,郑学仿.光谱法研究高铁肌红蛋白活性中心与咪唑的配位反应[J].无机化学学报,2011,27(3):445-450. |
| 作者姓名： | 周华伟 曹洪玉 唐 乾 李进京 郑学仿 |
| 作者单位： | 1. 辽宁省生物有机化学重点实验室,大连,116622 2. 大连大学生物工程学院,大连,116622;辽宁省生物有机化学重点实验室,大连,116622 3. 大连大学生物工程学院,大连,116622 |
| 基金项目： | 国家自然科学基金(No.20871024); 辽宁省高校创新团队项目(No.2006T002,2008T005,2009T003); 辽宁省教育厅项目(No.2009A069,2009A071); 大连市科技计划项目(No.2008E11SF170)资助 |
| 摘 要： | 采用紫外-可见(UV-Vis)、常规荧光、同步荧光等光谱法研究了高铁肌红蛋白(metMb)与咪唑的轴向配位反应的光谱性质。结果表明，metMb-咪唑配位体系的形成使得metMb的Soret带和Q带均发生红移；并对7位和14位2个Trp残基微环境极性的影响是不同的；同时还引起metMb活性中心铁卟啉597 nm处荧光增强，并且体系荧光强度与加入咪唑的浓度成线性关系，由此计算了不同温度下反应的配位数、结合常数及热力学参数；metMb与咪唑配体之间的配位反应是按物质的量比1∶1进行的；温度升高不利于配位反应的进行；热力学参数表明，此配位反应是自发进行的，焓变是整个配位反应的主要动力。 |
| 关 键 词： | 高铁肌红蛋白 咪唑 配位反应 热力学参数 荧光光谱 |
Spectral Study on Coordination Reaction between MetMb and Imidazole |
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| ZHOU Hua-Wei,CAO Hong-Yu,TANG Qian,LI Jin-Jing and ZHENG Xue-Fang.Spectral Study on Coordination Reaction between MetMb and Imidazole[J].Chinese Journal of Inorganic Chemistry,2011,27(3):445-450. | |
| Authors: | ZHOU Hua-Wei CAO Hong-Yu TANG Qian LI Jin-Jing and ZHENG Xue-Fang |
| Institution: | Liaoning Key Laboratory of Bio-organic Chemistry, Dalian University, Dalian, Liaoning 116622, China,College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China; Liaoning Key Laboratory of Bio-organic Chemistry, Dalian University, Dalian, Liaoning 116622, China,College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China; Liaoning Key Laboratory of Bio-organic Chemistry, Dalian University, Dalian, Liaoning 116622, China,College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China and College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China; Liaoning Key Laboratory of Bio-organic Chemistry, Dalian University, Dalian, Liaoning 116622, China |
| Abstract: | The spectroscopic properties of coordination reaction between metMb and imidazole were investigated by means of UV-Vis absorption spectra,fluorescence spectra,and synchronous fluorescence spectra.The experimental results showed that the transitions of Soret and Q band shifted to lower energies due to the formation of metMb-imidazole complex;effect of the formation of metMb-imidazole complex on Trps at position 7 and 14 were different;the equation for calculating the binding constant of imidazole to metMb wa... |
| Keywords: | metmyoglobin imidazole coordination reaction thermodynamic property fluorescence spectrum |
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