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| 渗调修复黄瓜陈种子基因组DNA损伤的RAPD研究 | |
| 引用本文： | 黄瑶,乔爱民,孙敏,伍时华,孙一铭.渗调修复黄瓜陈种子基因组DNA损伤的RAPD研究[J].西南师范大学学报(自然科学版),2005,30(1):141-144. |
| 作者姓名： | 黄瑶 乔爱民 孙敏 伍时华 孙一铭 |
| 作者单位： | 1. 西南师范大学,生命科学学院,重庆,400715;广西工学院,生物与化学工程系,广西,柳州,545006 2. 仲恺农业技术学院,园艺系,广东,广州,510225 3. 西南师范大学,生命科学学院,重庆,400715 4. 广西工学院,生物与化学工程系,广西,柳州,545006 |
| 基金项目： | 广东省自然科学基金资助项目(001424). |
| 摘 要： | 用Vc，CaCl2，PEG及其组合对自然老化的黄瓜种子进行渗调处理，利用随机扩增多态性DNA(RAPD)技术对处理前后的种子基因组DNA多态性进行比较分析．结果表明：渗调作用可以修复黄瓜陈种子的DNA损伤．修复效果与种子贮藏时间有关，贮藏半年的种子渗调处理前后RAPD产物差异不明显，贮藏1～2a的陈种子修复的效果好，表现为分子量高的DNA重新合成，RAPD扩增条带增多，亮度增加；贮藏3a的陈种子修复效果不理想。 |
| 关 键 词： | 渗调修复 自然老化种子 DNA损伤 随机扩增多态性DNA |
| 文章编号： | 1000-5471(2005)01-0141-04 |
| 收稿时间： | 3/8/2004 12:00:00 AM |
| 修稿时间： | 2004年3月8日 |
RAPD Researches on Genomic DNA Damages and Its Osmoprimed Repairment of Cucumber Aged Seeds |
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| HUANG,Yao.RAPD Researches on Genomic DNA Damages and Its Osmoprimed Repairment of Cucumber Aged Seeds[J].Journal of Southwest China Normal University(Natural Science),2005,30(1):141-144. | |
| Authors: | HUANG Yao |
| Institution: | HUANG Yao~ |
| Abstract: | Cucumber seeds stored in different times were treated respectively by various compounds of Vc, CaCl_2 and PEG. RAPD was applied to DNA polymorphism analysis of aged and treated seeds of cucumber. The results showed that osmoprimed ways could repair the DNA damages of aged seeds and that the treated effects were related to store times. Osmoprimed treatment had no striking difference on RAPD bands of seeds stored in half a year; seeds stored from one to two years had been repaired effectively, which resulted in resynthesis of large molecular mass DNA as well as improvements of the number and luminosity of RAPD bands; seeds stored tree years could hardly be repaired. |
| Keywords: | osmoprimed repairment aged seed DNA damage random amplified pdymorphic DNA |
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