Reliable and facile method for preparation of solventless bilayer lipid membranes for electroanalytical investigations |
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| Authors: | Nikolelis D P Krull U J |
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| Institution: | Chemical Sensors Group, Department of Chemistry, Erindale Campus, University of Toronto, 3359 Mississauga Road North, Mississauga, Ontario, Canada LSL 1C6. |
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| Abstract: | Bilayer lipid membranes continue to be of interest as elements for development and investigation of chemically-modified electrodes and biosensors, yet also continue to be difficult to prepare and replicate with precision. A simplified and reliable technique for the rapid formation of solventless bilayer lipid membranes is described, and the method has been shown to produce membranes which nominally vary by only 5-10% with respect to ion conductivity. Methods for rapid physical and chemical characterization of these membranes for establishment of reproducibility and quality are given. |
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