Spectrophotometric study of the interaction of tetraphenylporphyrin tetrasulfonate (TPPS4) with proteins |
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Authors: | Li N Tong S Y |
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Institution: | Chemistry Department, Peking University, Beijing, 100871, China. |
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Abstract: | The interaction of water-soluble porphyrin TPPS(4) (tetraphenylporphyrin tetrasulfonate) with proteins in acidic solution was studied by spectrophotometry. The absorption spectra of TPPS(4)-protein complexes, the aggregation of TPPS(4) in acidic solution, and comparison of the absorption spectra of TPPS(4)-protein conjugate with that of the TPPS(4)-protein complex was investigated in detail. The effects of denaturants including urea and SDS were also examined. A mechanism was proposed that TPPS(4) would be distributed between microphase of protein and the aqueous solution and then aggregated in the microphase. |
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