Application of photochemical inhibition in flow injection systems: Determination of epinephrine and L-dopa |
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Authors: | Pérez-Ruíz T Martínez-Lozano C Tomás V Val O |
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Institution: | Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Murcia, Spain. |
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Abstract: | An automated procedure for the photochemical determination of epinephrine and L-dopa has been developed. It is based on the strong inhibition by these catecholamines on the photochemical reaction between phloxin and ethylenediaminetetraacetic. The proposed flow-injection method allows the fluorimetric determination of epinephrine in the range 1.9-26.4 mug/ml and of L-dopa in the range of 1.5-12.7 mug/ml, with a sampling frequency of 35 samples/hr. The method was applied successfully to the determination of body catecholamines in pharmaceutical preparations. |
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