Simultaneous determination of ruthenium, osmium and palladium by reversed-phase HPLC using 4-(2'-thiazolylazo) resacetophenone oxime as chelating reagent

The chelates of Ru, Os and Pd with 4-(2'-thiazolylazo) resacetophenone oxime were separated simultaneously by HPLC using a pre-column derivatization method, at the wavelength 560 nm, on an octadecly-bonded silica stationary phase with a mobile phase methanol-water mixture (40-60 v/v) containing 40 mM of acetate buffer pH 5.0. The detection limits for Ru, Os and Pd were 2.0, 4.0 and 0.2 ng, respectively. The method has been applied successfully for the determination of metal ions in an anode slime.