Fabrication of microchip based on UV transparent polymer for DNA electrophoresis by F2 laser ablation |
| |
| Authors: | K Obata K Sugioka N Shimazawa K Midorikawa |
| |
| Institution: | (1) RIKEN (The Institute of Physical and Chemical Research), Hirosawa 2-1, Wako, Saitama 351-0198, Japan;(2) Brain Science Institute (BSI), RIKEN, Hirosawa 2-1, Wako, Saitama 351-0198, Japan |
| |
| Abstract: | A microchip for DNA electrophoresis made of a new UV transparent polymer material (CYTOP) whose absorption edge is much shorter than 190 nm has been fabricated by F2 laser ablation. F2 laser ablation achieves high-quality microfabrication of CYTOP surface with little deterioration of the optical property and little debris deposition at etched area. The microchip in which the microchannel connected with two reservoirs was embedded was successfully fabricated by bonding the ablated sample to the virgin CYTOP sheet. The fabricated microchip was applied for DNA analysis by electrophoresis and succeeded in separating different base-pares (bp) of DNA from 50 to 766 bp with resolution of 100 bp. PACS 42.70.Jk; 52.38.Mf; 82.45.-h |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
