高效液相色谱法测定农药拌种剂中的马钱子碱

 采用高效液相色谱法测定了农药拌种剂中马钱子碱的含量。色谱柱为 Spherisorb C18柱，以甲醇-水-溴代十六烷基三甲铵溶液(质量比为88∶12∶0.04）为流动相，检测波长为254 nm。在上述条件下，待测组分与其他组分达到基线分离。方法的线性范围为0.01 g/L～0.25 g/L,相关系数为0.999 7，回归方程为A=11 485 814ρ＋21 229，相对标准偏差为1.2%（n=11）。方法的稳定性高，重现性好，操作简便迅速，结果准确，是控制农药拌种剂内在质量的新方法。

Determination of Brucine in Seed Dressing Agents by High Performance Liquid Chromatography

An HPLC method for the determination of brucine in seed dressing agents was investigated. The sample solution was separated on a Spherisorb C18 column(4.6 mm i.d. x 200 mm, 5 microns) with CH3OH-H2O-CTAB (88:12:0.04, m/m) as the mobile phase and detected at 254 nm. Calibration curve of brucine was obtained for the concentration range of 0.01 g/L-0.25 g/L. The linear regression equation was A = 11,485,814 rho + 21,229, r = 0.9997. The RSD was 1.2%(n = 11). The results show that this method is simple, specific and accurate.