Pr?chromatographische in situ-Derivatisierung von Fetts?uren im Picomol-Bereich |
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| Authors: | A Junker-Buchheit und H Jork |
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| Institution: | (1) Fachbereich Pharmazie und Biologische Chemie, UniversitÄt des Saarlandes, D-6600 Saarbrücken, Bundesrepublik Deutschland |
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| Abstract: | Summary A simple and rapid derivatization method for C24-C6 fatty acids on HPTLC RP-18 phases is described. The prechromatographic reaction with monodansylpiperazine and monodansylcadaverine takes place at the start. Linear calibration curves are obtained after chromatography (e.g. dansyl palmityl piperazide: r=0.9998), which can be employed for quantitative analysis in the lower nanogram range (determination limit <10 ng). All 10 fatty acids can be separated excellently (R usually 1.5). Stepwise and gradient developments are employed (AMD system).This is the first time that in situ derivatization techniques have been employed for fatty acids leading to fluorescent products in the picomole range and a gradient method has been described which also leads to precise separations on RP phases.
Herrn Prof. Dr. W. Fresenius zu seinem 75. Geburtstag in Dankbarkeit gewidmet |
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