四川老坛泡菜中乳酸菌的分离与鉴定

老坛泡菜比一般泡菜具有更好的菌种多样性和菌群稳定性. 为筛选乳酸菌菌株, 从四川省眉山市仁寿县新安村农家坛龄达30年以上的老坛泡菜中, 分离得到12株疑似乳酸菌, 进行形态学观察、生理生化实验鉴定, 进一步利用16S rRNA基因序列比对、构建系统发育树, 确定疑似乳酸菌菌种. 分离鉴定出5株植物乳杆菌、3株短乳杆菌、2株肠膜明串珠菌、1株干酪乳杆菌和1株屎肠球菌, 其中以植物乳杆菌数量最多.

Isolation and identification of lactic acid bacteria from old jar pickles in Sichuan province

The stability and diversity of bacteria from the pickles marinated in old jars are better than those in normal jars. In the current study, the old-jar pickles were more than 30 years old and obtained from Xin-an Village, Renshou County, Meishan City, Sichuan Province. The pickles were used to screen lactic acid bacteria (LAB) with good properties. Twelve strains of LAB were isolated from the old-jar pickles and were identified by morphological observation, physiological and biochemical test, 16S rRNA gene sequence alignment and phylogenetic analysis. Five strains of Lactobacillus plantarum, 3 strains of Lactobacillus brevis, 2 strains of Leuconostoc mesenteroides, 1 strain of Lactobacillus casei and 1 strain of Enterococcus faecium were isolated and identified as predominant bacteria, while Lactobacillus plantarum was dominant among these 5 species of LAB.