Spherocyte shape transformation and release of tubular nanovesicles in human erythrocytes |
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Authors: | Iglic Ales Veranic Peter Jezernik Kristijan Fosnaric Miha Kamin Boris Hägerstrand Henry Kralj-Iglic Veronika |
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Affiliation: | Laboratory of Applied Physics, Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, SI-1000 Ljubljana, Slovenia. slrd.iglic@fe.uni-lj.si |
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Abstract: | We have studied dodecylmaltoside-induced echinocyte-spheroechincyte-spherocyte shape transformation and membrane vesiculation using transmission electron microscopy (TEM) on freeze-fracture replicas. It is indicated that spherical erythrocyte shape at higher dodecylmaltoside concentration is formed due to loss of membrane in the process where small, mostly tubular nanovesicles are released predominantly from the top of echinocyte and spheroechinocyte spicules. |
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