| 免疫亲和毛细管电泳的研究进展 |
| |
| 引用本文： | 陈泓序,张新祥. 免疫亲和毛细管电泳的研究进展[J]. 色谱, 2009, 27(5): 631-641 |
| |
| 作者姓名： | 陈泓序 张新祥 |
| |
| 作者单位： | Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
| |
| 基金项目： | 国家自然科学基金项目，科技部国家科技支撑计划项目 |
| |
| 摘 要： | 免疫亲和毛细管电泳方法结合了免疫分析的高特异性和毛细管电泳分离的高效、快速、样品用量少等优点,是复杂样品中特定组分分析的重要方法之一。激光诱导荧光检测器的使用以及毛细管电泳分离前免疫预富集过程的引入,可以进一步提高分析测定的灵敏度,使其能够用于痕量物质的高灵敏测定。本文结合作者所在课题组的工作,对免疫亲和毛细管电泳的两种主要模式,即均相的毛细管电泳免疫分析(CEIA)和非均相的免疫亲和毛细管电泳(IACE)的研究进展进行了综述。
|
| 关 键 词： | 毛细管电泳 免疫分析 免疫亲和 综述 |
| 收稿时间： | 2009-06-28 |
| 修稿时间： | 2009-07-15 |
Development of immunoaffinity capillary electrophoresis |
| |
| CHEN Hongxu,ZHANG Xinxiang. Development of immunoaffinity capillary electrophoresis[J]. Chinese journal of chromatography, 2009, 27(5): 631-641 |
| |
| Authors: | CHEN Hongxu ZHANG Xinxiang |
| |
| Affiliation: | Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
| |
| Abstract: | Immunoaffinity capillary electrophoresis is one of the important analytical technologies for complicated samples. It combines the advantages of both immunoassay and capillary electrophoresis, such as high specificity, high separation efficiency, rapid and low consumption. With the aid of laser-induced fluorescence detector and immuno-enrichment, the sensitivity of the method can meet the requirement for trace detection. In this paper, the research progress of homogenous capillary electrophoresis immunoassay (CEIA) and immunoaffinity capillary electrophoresis (IACE) is reviewed based on the research of the author’s group. |
| |
| Keywords: | capillary electrophoresis (CE) immunoassay immunoaffinity review |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《色谱》浏览原始摘要信息 |
|
点击此处可从《色谱》下载全文 |
|
