LC-MS/MS screening method for designer amphetamines, tryptamines, and piperazines in serum |
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Authors: | Ariane Wohlfarth Wolfgang Weinmann Sebastian Dresen |
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Institution: | 1. Institute of Forensic Medicine, University Medical Centre Freiburg, Albertstra?e 9, 79104, Freiburg, Germany
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Abstract: | Since the late 1990s and early 2000s, derivatives of well-known designer drugs as well as new psychoactive compounds have
been sold on the illicit drug market and have led to intoxications and fatalities. The LC-MS/MS screening method presented
covers 31 new designer drugs as well as cathinone, methcathinone, phencyclidine, and ketamine which were included to complete
the screening spectrum. All but the last two are modified molecular structures of amphetamine, tryptamine, or piperazine.
Among the amphetamine derivatives are cathinone, methcathinone, 3,4-DMA, 2,5-DMA, DOB, DOET, DOM, ethylamphetamine, MDDMA,
4-MTA, PMA, PMMA, 3,4,5-TMA, TMA-6 and members of the 2C group: 2C-B, 2C-D, 2C-H, 2C-I, 2C-P, 2C-T-2, 2C-T-4, and 2C-T-7.
AMT, DPT, DiPT, MiPT, DMT, and 5MeO-DMT are contained in the tryptamine group, BZP, MDBP, TFMPP, mCPP, and MeOPP in the piperazine
group. Using an Applied Biosystems LC-MS/MS API 365 TurboIonSpray it is possible to identify all 35 substances. After addition
of internal standards and mixed-mode solid-phase extraction the analytes are separated using a Synergi Polar RP column and
gradient elution with 1 mM ammonium formate and methanol/0.1% formic acid as mobile phases A and B. Data acquisition is performed
in MRM mode with positive electro spray ionization. The assay is selective for all tested substances. Limits of detection
were determined by analyzing S/N-ratios and are between 1.0 and 5.0 ng/mL. Matrix effects lie between 65% and 118%, extraction efficiencies range from 72%
to 90%. |
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