SQUID HYPSORHODOPSIN |
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| Authors: | Yoshinori,Shichida ,Fumio,Tokunaga Tó ru,Yoshizawa |
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| Affiliation: | Department of Biophysics, Faculty of Science, Kyoto University, Kyoto, Japan |
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| Abstract: | Abstract— Squid hypsorhodopsin is produced by irradiating rhodopsin or isorhodopsin with yellow light (>480nm) at liquid He temperature (4K). Compared with cattle rhodopsin, squid rhodopsin easily converts to a photosteady state mixture composed of rhodopsin, isorhodopsin, hypsorhodopsin and bathorhodopsin at this temperature and the amount of hypsorhodopsin in the mixture is high. Hypsorhodopsin has a main absorption peak at 446 nm, and its extinction coefficient is 1.16 times larger than that of rhodopsin. On warming above 35 K, squid hypsorhodopsin converts to bathorhodopsin. A kinetic analysis indicates that the hypsorhodopsin can be formed not only from rhodopsin but also from isorhodopsin. On absorption of light. both squid bathorhodopsin and hypsorhodopsin convert to a mixture of rhodopsin and isorhodopsin. |
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