高效液相法测定芦荟胶囊中的芦荟甙

 建立了高效液相法测定芦荟胶囊中芦荟甙含量的方法。实验采用ODS柱 ,以甲醇 水 (体积比为 5 0∶5 0 )溶液为流动相 ,用紫外检测器于 2 98nm处检测。结果表明 ,芦荟甙的进样量为 0 0 2 μg～ 1 5 μg时 ,进样量与峰面积呈良好的线性关系 (r =0 9999) ;样品的加标回收率为 99 0 %～ 10 1 9% ;方法的精密度好 ,相对标准偏差(RSD)为 1 37% (n =6 )。方法快速、简便、准确 ,所测结果稳定、重现性好 ,可作为胶囊类保健食品质量检验的一个定量方法。

Quantitative Determination of Barbalion in Aloe Capsule by High Performance Liquid Chromatography

Barbaloin in aloe capsule was quantitatively determined by high performance liquid chromatography with ODS column, a mixture of CH3OH-H2O (50:50, volume ratio) as mobile phase and UV detection at 298 nm. There was a good linear relationship within the range of 0.02 microgram-1.50 micrograms (r = 0.9999). The recovery was 99.0%-101.9% and the relative standard deviation was 1.37%(n = 6). The detection limit was 0.002 microgram (S/N = 2:1). The total time for separation and determination was within 10 min. The method is rapid, simple, accurate and reproducible. The method is proposed to be used for quality control of aloe capsule.