| HPLC测定河套大黄中的土大黄苷含量 |
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| 引用本文： | 陈志强,李贞景,傅志良,王昌禄. HPLC测定河套大黄中的土大黄苷含量[J]. 分析试验室, 2009, 28(1) |
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| 作者姓名： | 陈志强 李贞景 傅志良 王昌禄 |
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| 作者单位： | 1. 天津科技大学食品工程与生物技术学院,天津,300457;天津市尖峰天然产物研究开发有限公司,天津,300457
2. 天津科技大学食品工程与生物技术学院,天津,300457 |
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| 基金项目： | 天津科技大学引进人才科研启动基金 |
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| 摘 要： | 从河套大黄中分离纯化出活性成分土大黄苷,作为自制的标准品,利用KromasilTM C(18)(250 mm×4.6 mm I.d.,5μm)反相色谱柱,以V(甲醇):V(乙腈):V(水)=35:5:60为流动相,以320mm为检测波长,对河套大黄中土大黄苷进行测定.土大黄苷在0.427～4.190μg范围内与峰面积呈良好线性关系(r=0.9994),平均回收率为99.12%.该方法为河套大黄的质量控制奠定了基础.
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| 关 键 词： | 河套大黄 土大黄苷 高效液相色谱 |
Study on determination of rhaponticin in Rheum hotaoense C.Y.Cheng et C.T.Kao by HPLC |
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| CHEN Zhi-qiang,LI Zhen-jing,FU Zhi-liang,WANG Chang-lu. Study on determination of rhaponticin in Rheum hotaoense C.Y.Cheng et C.T.Kao by HPLC[J]. Chinese Journal of Analysis Laboratory, 2009, 28(1) |
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| Authors: | CHEN Zhi-qiang LI Zhen-jing FU Zhi-liang WANG Chang-lu |
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| Affiliation: | 1;2;1.College of Food Engineer and Biotechnology;Tianjin University of Science & Technology;Tianjin 300457;2.Jianfeng Natural Product Co.Ltd;Tianjin 300457 |
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| Abstract: | Rhaponticin which is the effective constituent of Rheum hotaoense C.Y.Cheng et C.T.Kao was isolated and identified.The rhapontiein standard samples were made by ourselves.A high performance liquid chromatographic method has been developed for the determination of rhaponticin in Rheum hotaoense C.Y.Cheng et C.T.Kao.The isocratic elution of methanol-acetonitrile-water (35:5:60) was used as the mobile phase.The UV detection wavelength was set at 260 nm.The calibration curve was linear in the range from 0.427 t... |
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| Keywords: | Rhapontineum hotaoense C.Y.Cheng et C.T.Kao. Rhaponticin HPLC |
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