SV40 INDUCTION FROM A MAMMALIAN CELL LINE BY ULTRAVIOLET RADIATION and THE PHOTOSENSITIZERS 8-METHOXYPSORALEN and ANGELICIN |
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Authors: | Sharon P Moore Howard Blount Thomas P Coohill |
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Institution: | Western Kentucky University, Bowling Green, KY 42101, USA |
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Abstract: | Abstract SV40-transformed weanling Syrian hamster kidney cells were exposed to long wavelength ultraviolet radiation in the presence of 8-methoxypsoralen or angelicin. The number of viruses induced as a result of this treatment was quantitated on CV-1P cells. The results indicate that angelicin, which is generally believed to form only monoadducts, is a potent viral inducing agent even when compared with 8-methoxypsoralen (8-MOP), which forms both monoadducts and crosslinks. At low radiation exposures 8-MOP is more effective than angelicin; as the radiation exposure is increased angelicin is capable of inducing the same peak level of virus expression as was 8-MOP. It appears that the induction of SV40 is a phenomenon possibly related to the repair of monoadducts. |
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