| 高效液相色谱-串联质谱法检测花生中的黄曲霉毒素B_1 |
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| 引用本文： | 杨静,哈益明,王锋.高效液相色谱-串联质谱法检测花生中的黄曲霉毒素B_1[J].分析试验室,2009,28(6). |
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| 作者姓名： | 杨静 哈益明 王锋 |
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| 作者单位： | 1. 中国农业科学院农产品加工研究所,北京,100193
2. 中国农业科学院农产品加工研究所,北京100193;农业部辐照产品监督检验测试中心,北京100193 |
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| 基金项目： | 国家科技支撑计划，和农业部公益性行业科研专项基金 |
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| 摘 要： | 应用高效液相色谱-电喷雾串联四极杆质谱联用系统(HPLC-MS/MS),在多反应离子检测方式(MRM)下,对花生中的黄曲霉毒素B1进行检测.对花生中黄曲霉毒素B1的提取、净化、液相分离及串联质谱等相关检测参数进行了优化研究.用V(甲醇):V(水)=6:4提取,OASIS HLB SPE小柱净化,定容过滤.采用V(甲醇):V(水)(含体积分数0.1%甲酸)=7:3为流动相,前级离子313.0,二级离子241.1、269.1,ESI正离子方式检测,在3.3 min出峰.结果表明,在ESI正离子模式下,黄曲霉毒素B1在其线性定量范围0.1～50μg/kg内,相关系数达到0.9999,检出限为0.03μg/kg,最低定量限为0.1μg/kg.低、中、高浓度添加回收率范围为93%～105%.
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| 关 键 词： | 黄曲霉毒素B1 高效液相色谱串联质谱 多离子反应检测 |
Determination of aflatoxin B_1 in peanuts by liquid chromatography-tandem mass spectrometry |
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| YANG Jing,HA Yi-ming,WANG Feng.Determination of aflatoxin B_1 in peanuts by liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Analysis Laboratory,2009,28(6). |
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| Authors: | YANG Jing HA Yi-ming WANG Feng |
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| Institution: | 1.Institute of Agro-Food Science & Technology;Chinese Academy of Agricultural Sciences;Beijing 100193;2.Quality Inspection and Test Center of Irradiated Products of Ministry of Agriculture;Beijing 100193 |
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| Abstract: | An analytical method for the determination of aflatoxin B1 in peanuts by high performance liquid chromatography-tandem mass spectrometry has been developed.This article focused on the optimization of extraction,clean-up,HPLC separation and MS/MS parameters of the analyte.Aflatoxin B1 was extracted by 60%(V/V) of methanol aqueous solution,and then it was purified by OASIS HLB SPE column.The eluted extract was analyzed by HPLC-MS/MS in positive ion mode using multiple reaction monitoring with a triple-quadrup... |
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| Keywords: | Aflatoxin B1 HPLC-MS/MS Multiple reaction monitoring |
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