| 磁性微球与牛血清白蛋白的相互作用 |
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| 引用本文： | 丁玲,李曦,张超灿. 磁性微球与牛血清白蛋白的相互作用[J]. 化学研究, 2010, 21(1): 19-22 |
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| 作者姓名： | 丁玲 李曦 张超灿 |
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| 作者单位： | 武汉科技大学,化学工程与技术学院,湖北省煤转化与新型炭材料重点实验室,湖北,武汉,430081;武汉大学,资源与环境科学学院,湖北,武汉,430079;武汉理工大学,理学院,湖北,武汉,430070;武汉理工大学,材料科学与工程学院,湖北,武汉,430070 |
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| 摘 要： | 以纳米级四氧化三铁为磁性载体,以苯乙烯为单体,用微悬浮聚合法制备了聚苯乙烯磁性微球;以牛血清白蛋白(BSA)为模型蛋白,用荧光光谱仪和紫外-可见吸收光谱仪研究了磁性微球与BSA的相互作用.结果表明,磁性微球与BSA结合反应的猝灭机理为静态猝灭.
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| 关 键 词： | 磁性微球 牛血清白蛋白 相互作用 |
Interaction of Polystyrene Magnetic Microspheres with Bovine Serum Albumin |
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| DING Ling,LI Xi,ZHANG Chao-can. Interaction of Polystyrene Magnetic Microspheres with Bovine Serum Albumin[J]. Chemical Research, 2010, 21(1): 19-22 |
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| Authors: | DING Ling LI Xi ZHANG Chao-can |
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| Affiliation: | 1 .College of Chemical Engineering and Technology, Hubei Provincial Key Laboratory of Coal Conversion and Advanced Carbon Material, Wuhan University of Science and Technology, Wuhan 430081, Hubei, China ; 2.School of Sciences, Wuhan University of Technology, Wuhan 430070, Hubei, China 3 .School of Materials Science and Engineering, Wuhan Uni＇oersity of Technology, Wuhan 430070, Hubei, China 4 .School of Resource and Environmental Science, Wuhan University, Wuhan 430079, Hubei, China ) |
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| Abstract: | Magnetic polystyrene microspheres were prepared via microsuspension polymeriza- tion by using Fe3O4 nanoparticles as magnetic carrier and styrene as monomer. The interaction of the magnetic microspheres with bovine serum albumin （BSA） was studied by means of fluorescence spectrometry and ultraviolet-visible spectrophotometry. Results show that the interaction of the magnetic microspheres with BSA is quenched by a static quenching mechanism. |
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| Keywords: | magnetic microspheres bovine serum albumin interaction |
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