Photochemical DNA activation |
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| Authors: | Lusic Hrvoje Young Douglas D Lively Mark O Deiters Alexander |
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| Institution: | Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA. |
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| Abstract: | A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function. |
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