Optimization of glucoamylase production by Aspergillus niger in solid-state fermentation |
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Authors: | Silvana T Silveira Melissa S Oliveira Jorge A V Costa Susana J Kalil |
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Institution: | (1) Department of Chemistry, Federal University Foundation of Rio Grande, 96201-900 Rio Grande, RS, Brazil |
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Abstract: | Glucoamylase production by Aspergillus niger in solid-state fermentation was optimized using factorial design and response surface techniques. The variables evaluated
were pH and bed thickness in tray, having as response enzyme production and productivity. The bed thickness in tray was the
most significant variable for both responses. The highest values for glucoamylase production occurred using pH 4.5 and bed
thickness in the inferior limits at 2.0–4.2 cm. For productivity, the optimal conditions were at pH 4.5 as well and bed thickness
from 4.4 to 7.5 cm. The optimal conditions for glucoamylase production while obtaining high activity without loss of productivity
were pH 4.5 and bed thickness in tray from 4.0 to 4.5 cm, which resulted in an enzyme production of 695 U/g and productivity
of 5791 U/h. |
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Keywords: | Aspergillus niger factorial design glucoamylase optimization solid-state fermentation |
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