Matrix effects and selectivity issues in LC-MS-MS |
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Authors: | J Smeraglia S F Baldrey D Watson |
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Institution: | (1) Department of Analytical Chemistry, University of Pardubice, 532 10 Pardubice, Czech Republic |
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Abstract: | Summary Chlorobenzenes, triazine and phenylurea herbicides were separated by normal micellar electrokinetic chromatography (MEKC)
and by micellar electrokinetic chromatography with reversed flow (RF-MEKC) in running buffers containing organic solvents.
The relationship between the two techniques is similar to that between reversed-phase and normal-phase HPLC. Using RF-MEKC,
the separation of lipophilic compounds is often improved compared to normal MEKC.
The migration in MEKC and in RF-MEKC was characterised by lipophilic and polar indices. The experimental values of the lipophilic
indices of the compounds tested in the two techniques were close to the indices in reversed-phase HPLC (RP-HPLC). This enables
the use of the indices determined in RP-HPLC for predicting the effects of changing composition of the running buffers on
migration times in MEKC and in RF-MEKC.
Presented at Balaton Symposium '01 on High-Performance Separation Methods, siófok, Hungary, September 2–4, 2001 |
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Keywords: | Column liquid chromatography Micellar electrokinetic chromatography Electroosmotic flow Retention indices Chlorobenzenes triazine and phenylurea herbicides |
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