Retention of Some Five-Membered Heterocyclic Compounds on a Porous Graphitized Carbon, Hypercarb™

The chromatographic performance of a Hypercarb^™ column was studied using some five-membered heterocyclic compounds. Significant retention of these molecules was observed with water/acetonitrile eluents. The influence of the organic modifier on retention was studied and interpreted on the basis of intermolecular interactions. The order of retention of solutes on Hypercarb^™ does follow their polarity order. Dispersion interactions undoubtedly have an influence, but do not determine the elution order of solutes. From the results obtained, it appears that the retention of the investigated substances is defined by interaction of polarized or polarizable functional groups in the samples with the graphite surface of the Hypercarb^™.