One-step to get 5-azidomethyl-2′-deoxyuridine from 5-hydroxymethyl-2′-deoxyuridine and detection of it through click reaction |
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| Authors: | Xiaowei Xu Shengyong Yan Jianlin Hu Pu Guo Lai Wei Xiaocheng Weng Xiang Zhou |
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| Institution: | 1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan 430072, PR China;2. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, PR China |
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| Abstract: | Nowadays a few ways to synthesize 5-azidomethyl-2′-deoxyuridine from 5-hydroxymethyl-2′-deoxyuridine have been reported. But none of them was one-step. And many of them need to protect the hydroxyl group on the pentose ring. The detection of 5-hydroxymethyl-2′-deoxyuridine is also very important in many biological processes. However few fluorescence detection strategies have been tried to do this. Herein, we reported a one-step protocol to synthesize 5-azidomethyl-2′-deoxyuridine, which was then used for detecting 5-hydroxymethyl-2′-deoxyuridine through a click reaction. |
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| Keywords: | 5-Hydroxymethyl-2&prime -deoxyuridine Click reaction Fluorescence One-step Detection |
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