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TRANSTHYRETIN (PREALBUMIN) GENE IN HUMAN PRIMARY HEPATIC CANCER
作者姓名:顾建人  蒋惠秋  贺丽萍  李岱宗  周筱梅  戴卫列  钱莲芳  陈渊卿  C. SCHWEINFEST  T. PAPAS
作者单位:Notional Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032,PRC,Notional Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032,PRC,Notional Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032,PRC,Notional Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032,PRC,Notional Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032,PRC,Notional Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032,PRC,Notional Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032,PRC,Notional Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032,PRC,Laboratory of Molecular Oncology,National Cancer Institute(NCI-FCRDC),USA,Laboratory of Molecular Oncology,National Cancer Institute(NCI-FCRDC),USA
摘    要:From a subtracting cDNA library constructed from normal liver versus human primary hepatic cancer (PHC), a cDNA clone pG8 was isolated. Using it as a probe, RNA extracted from one human liver and 9 PHC samples were analyzed by Northern hybridization. As expected, its mRNA was highly expressed in liver; however, the expression was strikingly suppressed in PHC. Only weak signal was observed in 2 out of 9 PHC, while no signal was detectable in the other 7 samples. Utilizing pG8 as a probe, DNA from the same PHC specimens was analyzed after MspI digestion and Southern hybridization. Deletion of DNA fragment was observed in 4 out of 9 samples. In further study of cancer and non-cancerous liver from other 7 PHC patients, similar deletion of DNA fragments in cancer was observed in 4 out of 7 samples. After sequencing of the clone of 572 bp, it was unexpectedly found that pG8 was completely homologous to the coding sequence of transthyretin, TTR gene, as TTR (or prealbumin) gene has been known to be linked to a


TRANSTHYRETIN (PREALBUMIN) GENE IN HUMAN PRIMARY HEPATIC CANCER
GU JIAN-REN JIANG HUI-QIU HE LI-PINGLI DAI-ZONG ZHOU XIAO-MEI DAI WEI-LIEQIAN LIAN-FANG CHEN YUAN-QING.TRANSTHYRETIN (PREALBUMIN) GENE IN HUMAN PRIMARY HEPATIC CANCER[J].Science in China(Chemistry),1991(11).
Authors:GU JIAN-REN JIANG HUI-QIU HE LI-PINGLI DAI-ZONG ZHOU XIAO-MEI DAI WEI-LIEQIAN LIAN-FANG CHEN YUAN-QING
Abstract:From a subtracting cDNA library constructed from normal liver versus human primary hepatic cancer (PHC), a cDNA clone pG8 was isolated. Using it as a probe, RNA extracted from one human liver and 9 PHC samples were analyzed by Northern hybridization. As expected, its mRNA was highly expressed in liver; however, the expression was strikingly suppressed in PHC. Only weak signal was observed in 2 out of 9 PHC, while no signal was detectable in the other 7 samples. Utilizing pG8 as a probe, DNA from the same PHC specimens was analyzed after MspI digestion and Southern hybridization. Deletion of DNA fragment was observed in 4 out of 9 samples. In further study of cancer and non-cancerous liver from other 7 PHC patients, similar deletion of DNA fragments in cancer was observed in 4 out of 7 samples. After sequencing of the clone of 572 bp, it was unexpectedly found that pG8 was completely homologous to the coding sequence of transthyretin, TTR gene, as TTR (or prealbumin) gene has been known to be linked to a hereditary disorder, familial amyloidosis (FAP), and related to thyroxine transport and binding to retinol-RBP (the retinol binding protein) complex. This is the first report of a study on TTR in human primary hepatic cancer. Since TTR gene was strikingly suppressed in mRNA expression and possibly defective in its gene structure, it was strongly implicated that TTR might be an important gene marker or a candidate of anti-oncogene for human PHC. The biological activity of TTR gene is under study.
Keywords:hepatoma  subtracting cDNA library  suppression  deletion  
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