Isolation and preparative purification of microcystin variants |
| |
Authors: | Ramanan S Tang J Velayudhan A |
| |
Institution: | Department of Bioresource Engineering, Oregon State University, Corvallis 97331-3906, USA. |
| |
Abstract: | Preparative reversed-phase liquid chromatography was successfully used to purify two microcystins (microcystin LR and microcystin LA) from a cyanobacterial process waste. The separation protocol involved extraction of lyophilized cells by methanol, isolation and concentration by solid-phase extraction, and purification by reversed-phase HPLC. Milligram-level loading of microcystins was obtained on a solid-phase extraction cartridge packed with 0.5 g of C18 stationary phase. The separations were first carried out on an analytical column and then scaled-up to a preparative column. The microcystins were quantified by HPLC and enzyme-linked immunosorbent assay. A method to remove microcystins rapidly and economically from the cyanobacterial process waste is also described. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|