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| 荧光光谱法研究纳米硫化锌与牛血清白蛋白的相互作用 | |
| 引用本文： | 陈稚,吴都督,张爽,林晓,龙烁杭,施志海,吴锐彬. 荧光光谱法研究纳米硫化锌与牛血清白蛋白的相互作用[J]. 分析测试学报, 2011, 30(11): 1272-1275. DOI: 10.3969/j.issn.1004-4957.2011.11.013 |
| 作者姓名： | 陈稚 吴都督 张爽 林晓 龙烁杭 施志海 吴锐彬 |
| 作者单位： | 1. 广东医学院分析中心,广东东莞,523808 2. 广东医学院药学院,广东东莞,523808 3. 广东医学院医学检验学院,广东东莞,523808 |
| 基金项目： | 2010年广东省教育厅育苗工程资助项目；广东省卫生厅资助项目（B2009190）；东莞市高等院校科研机构资助项目（20091085254） |
| 摘 要： | 采用水相法合成了ZnS纳米颗粒,通过XRD及TEM技术对纳米ZnS进行了表征,结果表明纳米ZnS的粒径约为7～8 nm.利用荧光光谱考察了纳米ZnS与牛血清白蛋白(BSA)的相互作用,结果显示,两者的相互作用可导致BSA内源荧光猝灭,推测其猝灭机理为静态猝灭,结合常数Ka=1.73×105 L·mol-1,结合位点数n... |
| 关 键 词： | 纳米硫化锌 牛血清白蛋白 荧光光谱 相互作用 静态猝灭 |
Investigation on Interaction of Bovine Serum Albumin with ZnS Nanoparticles by Fluorescence Spectroscopy |
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| CHEN Zhi,WU Du-du,ZHANG Shuang,LIN Xiao,LONG Shuo-hang,SHI Zhi-hai,WU Rui-bin. Investigation on Interaction of Bovine Serum Albumin with ZnS Nanoparticles by Fluorescence Spectroscopy[J]. Journal of Instrumental Analysis, 2011, 30(11): 1272-1275. DOI: 10.3969/j.issn.1004-4957.2011.11.013 | |
| Authors: | CHEN Zhi WU Du-du ZHANG Shuang LIN Xiao LONG Shuo-hang SHI Zhi-hai WU Rui-bin |
| Affiliation: | CHEN Zhi1*,WU Du-du2,ZHANG Shuang3,LIN Xiao2,LONG Shuo-hang2,SHI Zhi-hai2,WU Rui-bin2(1.Center of Analysis,Guangdong Medical College,Dongguan 523808,China,2.School of Pharmacy,3.School of Laboratory Medicine,China) |
| Abstract: | ZnS nanoparticles was synthesized using zinc acetate and sodium sulfide as starting materials in water solution.The morphology and crystal phase of nano-ZnS were characterized by transmission electron microscopy(TEM) and powder X-ray diffraction(XRD).The interaction of nanoparticles and bovine serum albumin(BSA) was investigated by fluorescence spectroscopy in pH 7.0 Tris-HCl buffer.The result showed that the interaction between BSA and ZnS nanoparticles could resulted in the endogenous fluorescence quenchi... |
| Keywords: | nano-ZnS bovine serum albumin(BSA) fluorescence spectroscopy interaction static quenching |
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