| 一种聚甲基丙烯酸酯类亲水作用毛细管电色谱整体柱的制备与评价 |
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| 引用本文： | 黄桂华,陈思谨,林旭聪,谢增鸿.一种聚甲基丙烯酸酯类亲水作用毛细管电色谱整体柱的制备与评价[J].色谱,2010,28(12):1173-1178. |
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| 作者姓名： | 黄桂华 陈思谨 林旭聪 谢增鸿 |
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| 作者单位： | 1. 福建中医药大学药学院, 福建 福州 350108; 2. 福州大学食品安全与环境监测技术研究所, 福建 福州 350108 |
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| 基金项目： | 福建省自然科学基金项目(No. 2009J05072). |
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| 摘 要： | 以2-羟基乙基甲基丙烯酸酯(HEMA)为单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,制备了亲水分离模式的聚HEMA-co-EDMA新型毛细管电色谱(CEC)整体柱。考察了整体柱的结构特征及其CEC性能,研究了极性物质的保留行为,并对其可能的保留机理进行了探讨。所制备的整体柱稳定性好,固定相表面带有极性羟基功能团,不仅能提供亲水相互作用位点,且能吸附流动相中的阴离子产生阳极电渗流(EOF)。在流动相中乙腈含量较高(＞62%,体积分数)的条件下,整体柱表现出典型的亲水作用,实现了对核苷、碱基和苯胺类带有碱性的强极性化合物的高效分离,并成功分离了苯酚类、苯甲酸类等中性或酸性的极性化合物。
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| 关 键 词： | 聚合整体柱 毛细管电色谱 亲水作用 |
| 收稿时间： | 2010-09-03 |
Preparation and evaluation of a polymethacrylate based monolithic column for hydrophilic interaction capillary electrochromatography |
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| HUANG Guihua,CHEN Sijin,LIN Xucong,XIE Zenghong.Preparation and evaluation of a polymethacrylate based monolithic column for hydrophilic interaction capillary electrochromatography[J].Chinese Journal of Chromatography,2010,28(12):1173-1178. |
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| Authors: | HUANG Guihua CHEN Sijin LIN Xucong XIE Zenghong |
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| Institution: | 1. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China; 2. Institute of Food Safety and Environmental Monitoring, Fuzhou University, Fuzhou 350108, China |
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| Abstract: | A hydrophilic monolithic column was prepared by in-situ polymerization of 2-hydroxyethyl methacrylate(HEMA) as a functional monomer and ethylene glycol dimethacrylate(EDMA) as a cross-linking agent in the fused-silica capillary.The monolithic column was evaluated as a hydrophilic interaction capillary electrochromatography(HI-CEC) stationary phase for polar solutes.The polar sites on the surface of the monolithic stationary phase responsible for hydrophilic interactions were provided from the hydroxy groups... |
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| Keywords: | capillary electrochromatography (CEC) hydrophilic interaction polymer-based monolithic column |
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