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Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography |
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| Authors: | Jussi Meriluoto Brendan Kincaid Malcolm R Smyth and Mikael Wasberg |
| Institution: | a Department of Biochemistry and Pharmacy, Åbo Akademi University, Biocity, P.O. Box 66, FIN-20521 Turku, Finland b School of Chemical Sciences, Dublin City University, Dublin 9, Ireland c Laboratory of Analytical Chemistry, Åbo Akademi University, Piispankatu 8, FIN-20500 Turku, Finland |
| Abstract: | A novel amperometric HPLC detection method for the cyanobacterial (blue–green algal) peptide toxins microcystin-LR, -YR and -RR was developed. Purified microcystins and cyanobacterial extracts were chromatographed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reactions at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were shown to originate in arginine and tyrosine residues of microcystins. |
| Keywords: | Microcystins Hepatotoxins Toxins |
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