| 反相高效液相色谱法测定深层培养含硒构菌菌丝体中的硒蛋氨酸 |
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| 引用本文： | 章军,傅庭治.反相高效液相色谱法测定深层培养含硒构菌菌丝体中的硒蛋氨酸[J].分析化学,1995,23(1):49-51. |
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| 作者姓名： | 章军 傅庭治 |
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| 作者单位： | 南京大学生物科学与技术系 南京210093
(章军，傅庭治)，南京大学生物科学与技术系 南京210093(曹幼琴) |
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| 摘 要： | 本文用苯异硫氰酸酯作柱前衍生化试剂，衍生的硒蛋氨酸及其它非硒代氨基酸用反相高交液相色谱进行分离分析，分析表明，加无机硒培养的构菌菌丝体中含一定量的硒蛋氨酸，且这一方法广泛适于生物物质中其它硒代氨基酸的分析。
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| 关 键 词： | 硒蛋氨酸 硒 构菌 反相 高效液相色谱 |
Determination of Selenomethionine in Mycelia of Submerged Cultural Selenium Flammulina Velutipes by Reversed-phase High-performance Liquid Chromatography |
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| Zhang Jun,Fu Tingzhi,Cao Youqin.Determination of Selenomethionine in Mycelia of Submerged Cultural Selenium Flammulina Velutipes by Reversed-phase High-performance Liquid Chromatography[J].Chinese Journal of Analytical Chemistry,1995,23(1):49-51. |
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| Authors: | Zhang Jun Fu Tingzhi Cao Youqin |
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| Abstract: | A reversed-phase high-performance liquid chromatography for the separation of selenomethionine and amino acids was developed. In the method, phenyl-isothiocyanate was chosen as the reagent for the precolumn derivatization of selenomethionine and amino acids. These derivatives were efficiently separated on a C18 column. The analytical results'show that the selenomethionine contents in the mycelia of selenium flammulina velutipes associates with the concentration of selenium in the media as well as the kinds of media. This method is rapid, accurate and sensitive. |
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| Keywords: | Selenomethionine selenium flammulina velutipes reversed-phase high-performance liquid chromatography |
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