Comparative Evaluation of CE and HPLC for Determination of Cotinine in Human Urine |
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Authors: | Piotr Kowalski Marcin Marsza?? Ilona Ol?dzka Wojciech Czarnowski |
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Institution: | (1) Faculty of Pharmacy, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland |
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Abstract: | Two rapid and popular methods—capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) have been compared
for analysis of cotinine in human urine. Cotinine was analyzed in less than 7 min, with detection limits of 5 and 3.2 ng mL−1 for CE and HPLC, respectively. The performance of the methods was evaluated in terms of sensitivity, specificity, precision,
accuracy, and limits of detection and quantification. Calibration plots were linear in the range 50–4,000 ng mL−1, at least, and mean recoveries were satisfactory for both techniques. The methods were successfully used for quantification
of cotinine in urine. |
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Keywords: | Capillary electrophoresis Column liquid chromatography Human urine Cotinine Validation study |
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