Protein synthesis in cell-free reticulocyte lysates on multi-hour incubation |
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Authors: | Findeis Mark A Whitesides George M |
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Institution: | (1) Department of Chemistry, Harvard University, 02138 Cambridge, MA |
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Abstract: | Cell-free protein synthesis in rabbit reticulocyte lysate translation mixtures has been studied during multi-hour incubations.
In an impaired lysate obtained from cells stored at 0°C before lysis, and showing a low initial rate of synthesis, translation
could be stimulated by a factor of 4 by including RNase inhibitor and additional ATP and GTP. In translation mixtures prepared
from normal lysates, protein synthesis could be improved by ∼50% by the addition of excess GTP. The observed increases in
protein synthesis were obtained by improved maintenance of the initial rate of synthesis. |
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Keywords: | Cell-free protein synthesis cell-free translation reticulocyte lysate and behavior on multi-hour incubation |
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