Investigation of restriction endonuclease EcoRII complex with DNA in solution by FTIR spectroscopy |
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| Authors: | F V Subach J Liquier E S Gromova |
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| Institution: | (1) Albert Einstein Medical College, New York, USA;(2) Department Health, Medicine, and Human Biology, University Paris-13, Paris-13, France;(3) Lomonosov Moscow State University, Leninskie gory 1, str. 3, Moscow, 119991, Russia |
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| Abstract: | The X-ray structure of type IIE EcoRII restriction endonuclease has been solved but the structure of the R.EcoRII-DNA complex is still unknown. We report here on the structure of the pre-reactive R.EcoRII-DNA-Ca2+ complex in solution examined by FTIR spectroscopy. The secondary structure of R.EcoRII as well as the structure of the target DNA in the R.EcoRII-DNA-Ca2+ complex was characterized. It was shown that the R.EcoRII-DNA-Ca2+ complex formation is accompanied by changes in the spectrum of both DNA bases and DNA sugar-phosphate backbone that suggest contacts of the enzyme with different groups of atoms in DNA. The change of the R.EcoRII secondary structure in the R.EcoRII-DNA-Ca2+ complex is also observed. |
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