| 碱裂解法提取质粒DNA方法的改进 |
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| 引用本文： | 张学民,王金生.碱裂解法提取质粒DNA方法的改进[J].安徽大学学报(自然科学版),2000,24(4):75-78. |
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| 作者姓名： | 张学民 王金生 |
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| 作者单位： | 南京农业大学植保系农业部病虫监测与治理重点开放实验室，江苏南京 2l0095 |
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| 基金项目： | 国家高技术研究发展计划(863计划);863-101-03-02-01; |
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| 摘 要： | 对碱裂解法提取质粒DNA的方法进行了一些改进 ,在室温下裂解菌体和变性DNA ;用NH4 Ac和LiCl同步沉淀除去样品中的蛋白质和RNA ,降低了对超螺旋质粒的剪切作用 ,得到高质量的质粒DNA。
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| 关 键 词： | 碱裂解 NH_4Ac LiCl |
| 文章编号： | 1001-5019(2000)04-0075-04 |
A Modified Alkaline Lysis Method for the Preparation of Plasmid DNA |
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| ZHANG Xue-min,WANG Jin-sheng.A Modified Alkaline Lysis Method for the Preparation of Plasmid DNA[J].Journal of Anhui University(Natural Sciences),2000,24(4):75-78. |
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| Authors: | ZHANG Xue-min WANG Jin-sheng |
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| Abstract: | Modifications were made on the preparation of plasmid by alkaline lysis. Cells were lysed and DNA were denatured under room temperature, NH 4Ac and LiCl were both used to precipitate proteins and RNA in sample ,destruction of supercoiled DNA were reduced, which yield high quality plasmid DNA. |
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| Keywords: | NH4Ac LiCl alkaline lvsis NH4Ac LiCl |
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