Spectrofluorimetric determination of uric acid based on its activation of catalytic oxidation of pyronine Y |
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| Authors: | Suling Feng Xueping Liu |
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| Institution: | (1) College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, 453007, China |
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| Abstract: | A novel kinetic spectrofluorimetric method for the determination of uric acid based on the activation effect of uric acid
on the Cu(II) ion catalyzed oxidation of pyronine Y by hydrogen peroxide was developed. The influence of different buffer
solutions was tested and the Britton-Robinson buffer solution with pH 2.2 was found to be the optimum. The detection limit
and the linear range for uric acid are 0.09 μg mL−1 and 0.3–3.0 μg mL−1, respectively. The RSD for eleven determinations of 1.6 μg mL−1 uric acid was 1.6 %. Satisfactory results were obtained when using this method of uric acid determination in human urine. |
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| Keywords: | kinetic spectrofluorimetric method activation uric acid pyronine Y |
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