Magnetically Alignable Bicelles with Unprecedented Stability Using Tunable Surfactants Derived from Cholic Acid |
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| Authors: | Ryoichi Matsui Dr Noriyuki Uchida Dr Masataka Ohtani Kuniyo Yamada Arisu Shigeta Prof?Dr Izuru Kawamura Prof?Dr Takuzo Aida Dr Yasuhiro Ishida |
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| Institution: | 1. Department of Chemistry and Biotechnology, School of Engineering, the, University of Tokyo, Tokyo, Japan;2. RIKEN Center for Emergent Matter Science, Saitama, Japan;3. Graduate School of Engineering, Yokohama National University, Yokohama, Japan |
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| Abstract: | Five novel surfactants were prepared by modifying the three hydroxy groups of sodium cholate with triethylene glycol chains endcapped with an amide ( SC‐C1 , SC‐ n C4 , and SC‐ n C5 ) or a carbamoyl group ( SC‐O n C4 and SC‐O t C4 ). The phase behavior of aqueous mixtures of these surfactants with 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphatidylcholine (DMPC) was systematically studied by 31P NMR spectroscopy. The surfactants endcapped with carbamate groups ( SC‐O n C4 and SC‐O t C4 ) formed magnetically alignable bicelles over unprecedentedly wide ranges of conditions, in terms of temperature (from 21–23 to >90 °C), lipid/surfactant ratio (from 5 to 8), total lipid content (5–20 wt %), and lipid type DMPC, 1,2‐dilauroyl‐sn‐glycero‐3‐phosphatidylcholine (DLPC), or 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphatidylcholine (POPC)]. In conjunction with appropriate phospholipids, the carbamate‐endcapped surfactants afforded unique bicelles, characterized by exceptional thermal stabilities (from 0 to >90 °C), biomimetic lipid compositions (DMPC/POPC=25:75 to 50:50), and extremely large 2H quadrupole splittings (up to 71 Hz). |
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| Keywords: | bicelles cholic acid magnetic alignment phospholipids surfactants |
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