Analysis of saikosaponins in Bupleuri Radix and Caihu-shugan-san using reversed-phase HPLC with pulsed amperometric detection |
| |
Authors: | Kwon Ha-Jeong Sim Hee-Jung Lee Sa-im Lee Yong-Moon Lee Je-Hyun Park Yong-Duk Hong Seon-Pyo |
| |
Affiliation: | Department of Preventive and Social Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea. |
| |
Abstract: | A simple and sensitive reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD) method was developed to determine the saikosaponin content in Bupleuri Radix or Caihu-shugan-san. Four saikosaponins in Bupleuri Radix and Caihu-shugan-san were extracted with a 6:4 solution of 10 mM sodium phosphate buffer (pH 8)/100% ethanol. Pulsed amperometric detection of carbohydrates in four major saikosaponins was highly sensitive when used with a water-acetonitrile gradient on an alkaline RP column with a post-column delivery system. The limits of detection (S/N=3) and of quantification (S/N=10) of saikosaponins were 0.01-0.02 and 0.03-0.05 μg/mL, respectively. The intra- and inter-day precision (RSDs) were each <9.7% and the average recoveries were 95.0-97.6% in Bupleuri Radix. This method can be used to analyze saikosaponins in Bupleuri Radix and Caihu-shugan-san. |
| |
Keywords: | Pulsed amperometric detection Reversed‐phase HPLC Saikosaponins Stability |
本文献已被 PubMed 等数据库收录! |