SPECTRAL ANALYSIS OF CHLOROPHYLL a AGGREGATES IN THE PRESENCE OF WATER-SOLUBLE MACROMOLECULES |
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| Authors: | Kaku Uehara Mamoru Mimuro Yoshihiko Fujita Makoto Tanaka |
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| Affiliation: | Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture, Sakai, Osaka 591, Japan;National Institute for Basic Biology, Okazaki, Aichi 444, Japan |
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| Abstract: | Abstract— The absorption and fluorescence spectra of chlorophyll a (Chi a) aggregates formed in aqueous solutions of polyvinyl alcohol) (PVA), polyvinyl pyrrolidone) (PVP), and bovine serum albumin (BSA) were analyzed by curve-fitting methods in the wavelength region from 650 to 800 nm. The results indicated that the aggregation of Chi a to polymeric forms such as (Chia–2H20), was suppressed in the presence of the macromolecules. The suppression was due to a coordination of macromolecule bound ligands to Chi a and was strongest in BSA and weaker in PVA. There were differences in the spectra even though the same types of polymeric Chi a forms were observed due to characteristically different composition of these forms. Fluorescence patterns indicated that energy was transferred from the shorter to the longer wavelength forms. |
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