| 丝心蛋白基因分子克隆与表达的初步探讨 |
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| 引用本文： | 李宏杰,蔡再生,施华芳,于同隐,黄伟达. 丝心蛋白基因分子克隆与表达的初步探讨[J]. 高等学校化学学报, 1998, 19(4): 564-567 |
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| 作者姓名： | 李宏杰 蔡再生 施华芳 于同隐 黄伟达 |
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| 作者单位： | 1. 复旦大学生物化学系,上海,200433
2. 高分子科学系,上海,200433 |
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| 摘 要： | 通过聚合酶链反应扩增丝心蛋白Ｃ亚基结构的基因，并将基克隆到融合蛋白表达载体ｐＲＩＴ２Ｔ质粒中得到ｐＲＩＴ２Ｔ－ＦＬ质粒，在大肠杆菌株Ｐ２３９２内进行表达。十二烷基硫酸钠聚丙烯酰胺凝胶电泳和免疫印迹反应证明融合蛋白在大肠杆菌中得到了表达。
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| 关 键 词： | 丝心蛋白 C亚基 基因 克隆 表达 大肠杆菌 |
A Preliminary Study on Expression of Fibroin C Subunit as a Fusion Protein in E.Coli |
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| LI Hong-Jie,CAI Zai-Sheng,SHI Hua-Fang,YU Tong-Yin,HUANG Wei-Da. A Preliminary Study on Expression of Fibroin C Subunit as a Fusion Protein in E.Coli[J]. Chemical Research In Chinese Universities, 1998, 19(4): 564-567 |
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| Authors: | LI Hong-Jie CAI Zai-Sheng SHI Hua-Fang YU Tong-Yin HUANG Wei-Da |
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| Abstract: | The structural gene of fibroin C subunit(i.e. fibroin light chain, FL) was amplified by polymerase chain reaction(PCR) from pFL18 plasmid, which carries a cDNA clone of fibroin C subunit. The PCR product was cloned on fusion protein expression vector pRIT2T, and was expressed in the E. Coli strain P2392. SDS polyacrylamide gel electrophoresis(SDS PAGE) and immunoblotting were carried out to verify the expression of protein A fibroin C subunit fusion protein. |
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| Keywords: | Fibroin C subunit Fusion protein Protein A Immunoblotting |
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